Journal: Molecular Medicine Reports
Article Title: ULK1 activates NCOA4-mediated ferritinophagy via the Beclin1/VPS34 complex in cardiomyocyte hypertrophy
doi: 10.3892/mmr.2026.13826
Figure Lengend Snippet: ULK1 expression is elevated in hypertrophic cardiac tissues and cardiomyocytes. (A) C57BL/6J mice at 8-weeks-old were subjected to sham or TAC surgery. Representative whole heart images (scale bar, 0.5 cm), M-mode echocardiography, H&E staining (scale bar, 100 µm) and WGA staining (scale bar, 50 µm) of heart tissue. Assessment of (B) HW/BW, (C) LVW/BW and (D) HW/TL (n=6). Statistical graphs for (E) LVEF (%), (F) FS (%), (G) IVS, d (mm) and (H) LVPW, d (mm) (n=6). (I) Cardiomyocyte cross-sectional area acquired from WGA staining. Cells were measured from different microscopic fields of 6 samples in each group. The mRNA levels of (J) BNP and (K) β-MHC in the hearts from sham or TAC-surgery mice (n=6). The mRNA levels of (L) BNP and (M) β-MHC in HL-1 cells treated with Ang II (n=3). (N) Stained cells and (O) quantification of cell surface area of HL-1 cells treated with Ang II. Scale bar, 50 µm (n=3). Western blot analysis and summarized data demonstrating (P) Beclin1 and (Q) VPS34 protein expression in HL-1 cells treated with Ang II (n=3). (R) Western blot analysis and summarized data demonstrating ULK1 protein levels in the hearts obtained after sham or TAC surgery (n=6). (S) Western blot analysis and summarized data demonstrating ULK1 protein levels in the cardiomyocytes stimulated with Ang II (n=6). All data are presented as mean ± SEM. *P < 0.05 and **P<0.01 vs. sham or control group. ULK1, serine/threonine protein kinase ULK1; TAC, transverse aortic constriction; HW, heart weight; BW, body weight; LVW, left ventricular weight; TL, tibia length; LVEF, left ventricular ejection fraction; FS, fractional shortening; d, diastole; IVS, interventricular septal thickness; LVPW, left ventricular posterior wall thickness; CSA, cross-sectional area; BNP , brain natriuretic peptide; β-MHC , β-myosin heavy chain; α-SMA, α smooth muscle actin; Ang II, angiotensin II; VPS34, PI3K catalytic subunit type 3; WGA, wheat germ agglutinin.
Article Snippet: The Ang II AT1-receptor candesartan (CS; cat. no. HY-B0205) was purchased from MedChemExpress.
Techniques: Expressing, Staining, Western Blot, Control
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